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Mutation of Y223 to the phenylalanine residue resulted in the mutant with approximately a similar catalytic exercise since the wild form enzyme and so this residue will not be considerable to the binding of substrate in NagA.
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cat vs. pH profile, one ionization is observed for either Zn- or Cd-NagA. This ionization must signify the protonation of your steel-sure hydroxide that is used for substrate hydrolysis or, alternatively, the overall base which is used to deprotonate the metal-bound drinking water molecule just before substrate hydrolysis. The p
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Residues Q59 and N61 have been mutated with each other to the pair of histidine residues to make an HxH motif which is analogous to that located in NagA from B. subtilis
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